随着人造光的广泛应用，人周围的光环境也越来越复杂。频闪光刺激存在于多种人造光源当中，充斥于我们的日常生活，会对人的健康产生影响，且频闪光刺激对人生理状态的影响及其影响机制尚未明确。因此，本论文聚焦于不同频率的频闪光刺激，使用生理测量的研究方法，设计人体功效学临床试验，招募受试者，观察受试者在不同频闪光刺激下的皮肤电数据和心电图数据，对数据进行处理与分析，总结数据的变化规律，探究频闪光刺激的光神经生物效应。本论文的研究内容包括以下三个方面：（1）设计并完成了频闪光刺激试验系统和频闪光刺激临床试验，形成了一套研究频闪光刺激光生物效应的研究方法。本研究设计开发的频闪光刺激试验系统可以产生不同频率的频闪光刺激，并实时采集受试者的皮肤电和心电图数据。本研究还开展了预试验和频闪光刺激试验，采集受试者在不同频闪光刺激下的皮肤电数据和心电图数据，为探究频闪光刺激的光神经生物效应奠定基础。（2）对皮肤电数据和心电图数据进行数据处理，提取了相关数据特征。首先，本研究对皮肤电数据和心电图数据进行了频率滤波，然后设计实现了皮肤电反应定位算法提取了皮肤电数据的信号特征，且设计实现了心电图数据的R波定位算法分割心跳间期，引入心率变异性的时域分析和频域分析，提取了心电图数据的信号特征。（3）对皮肤电数据和心电图数据进行数据分析，研究频闪光刺激对皮肤电数据和心电图数据的影响。统计学分析结果表明一定频率的频闪光刺激会使人的皮肤电反应幅值升高和使人的平均心率上升、心率变异性时域指标下降和频域指标低频段功率上升，具有统计学意义。皮肤电数据和心电图数据都是交感神经系统的评估指标，皮肤电数据和心电图数据的数据结果较为一致地表明频闪光刺激可能会促进交感神经系统兴奋，进而影响人的生理状态。本研究通过研究频闪光刺激对皮肤电数据和心电图数据的影响，发现了频闪光刺激可能会通过促进交感神经系统兴奋来影响人的生理状态，拓展了频闪光刺激的光神经生物效应。
With the wide application of artificial light, the light environment around us is becoming more and more complicated. Flicker and the stroboscopic effect that have an impact on human health exist in a variety of artificial light sources and are filled with our daily life. Moreover, the influence of flicker and the stroboscopic effect on human health and its mechanism have not been clear. Therefore, this thesis is to explore the neurobiological effects of different flicker and the stroboscopic effect by designing human ergonomic clinical trial. The subjects were recruited, the electrodermal data and electrocardiogram data of subjects under different strobe stimulation were collected and the data were processed and analyzed. Then, we summarized the effects of flicker and the stroboscopic effect on electrodermal data and electrocardiogram data to inquiry the neurobiological effects of flicker and the stroboscopic effect. This thesis mainly includes the following three contents:(1) Firstly, we designed and completed the strobe stimulation experiment system and the strobe stimulation clinical trial, which formed a set of research methods to study the neurobiological effects of flicker and the stroboscopic effect. The strobe stimulation experiment system could generate different frequency strobe stimulation and synchronously collect electrodermal data and electrocardiogram data of subjects. Then, the pre-trial and the strobe stimulation clinical trial were conducted including recruiting subjects, collecting electrodermal data and electrocardiogram data of subjects under different strobe stimulation, which is the foundation for the following work.(2) Secondly, the electrodermal data and electrocardiogram data were processed, and their features were extracted. Firstly, the electrodermal data and electrocardiogram data were preprocessed by frequency filtering. Then, based on the theoretical characteristics of the electrodermal data, we designed a skin conductance response localization algorithm to locate the skin conductance responses and extracted data features of the electrodermal data. In addition, we designed a R-wave localization algorithm of electrocardiogram to segment the heartbeat interval. Then, time analysis indexes and frequency analysis indexes of heart rate variability were obtained. We also acquired the relevant features of electrocardiogram data.(3) Thirdly, the electrodermal data and electrocardiogram data were analyzed to study the effects of strobe stimulation on electrodermal data and electrocardiogram data. The statistical analysis results showed that flicker and the stroboscopic effect would significantly increase the amplitude of human electrodermal response and the average human heart rate, decrease the time analysis indexes of heart rate variability and increase the low-frequency power of the frequency analysis indexes of heart rate variability. Electrodermal data and electrocardiogram data are both related to the sympathetic nervous system, and the analysis results consistently indicated that flicker and the stroboscopic effect may promote the excitation of sympathetic nervous system to affect human physiological state.Through studying the effects of flicker and the stroboscopic effect on electrodermal data and electrocardiogram data, we found that flicker and the stroboscopic effect may affect human physiological state by promoting sympathetic nervous system excitation, and expanded the neurobiological effects of flicker and the stroboscopic effect.
